Elphidium margaritaceum Unknown

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Environmental Data
Principle Investigator(s)	Jan Pawlowski
External sample ID	12EM
NCGR Sample ID	MMETSP1385
Sample accession number	CAM_SMPL_003143
Assembly accession number	CAM_ASM_000861
Combined Assembly Name	N/A
Genus	Elphidium
Species	margaritaceum
Strain	Unknown
Clonal	No
Axenic	No
Prelim. NCBI Taxon ID	933848
18S rRNA	TATCAGTTTAAATATACGCGCAAGCGTACATGCAACTGCAGACAGCTGCTTAATACAGTCACACT TGTCTTGACTCGGTTTATATTTTCGATCATACGGCGCTCAACGGATAACTCAGGGAAAGTTTGGCTAATA CGTACGAACACACTTCTATTGTCGCATACAGTTACGCATGAATGAGATTGTATACGTGCGCATGAATTTA TTCATCGCACAAGGCAGATATTGTAATTTATTACGATACATGTCATACAAGCATCTACAGCATCAAGTCA CAGGGTTGGCAAGTGTATTTTTGAACCTTCAAAGCAGTCACGCATACGGAAGAGTAGTTTCTGATCCCAT AGAAGGAGCACTGTACATTGAGAGACCGCTCTTAGTTCTAAGGAACGCAGCAGGCGCGTAAATTGCCCAA TAATAGTACATACGGTATATTCACTCTCATTCTATTGAGGCAGTGATAAGCTGTAACGGTTGAGTATTAA TCTAATTTGACGTGTATCTGGCATGCGTTATACGTTTACGCGTATATTACTGCATACGCCGATACTATGT GTGCTCAGTCTGGAATGCGGTGAGTCTAAACAACTCAGAACCTATATTAATTTATGTATCTGAATTTTCA AGTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATACCAGCTCCACTGGMCTATACAATCATTGTTGC GGTTAAGAGGCTCGTAGTTGGATTGAACAACCACTATACACAATACTGTGATCAAATCAGCACGTATCAC GTATGTATTTTTTAATACGCATTGTATGTTTATTCATGGAATGTTGTATTTTTATACGTCGATGGGGATA GTTGGAGTCAACAGTACTAGTAGGCGAGCGGTGAAATGCATTGACCCTACCAAGACTACCAAAAGCGAAA GCAGTTGTCTAGGCTATATTCTTTATACAGTACTACACGTACACGTATACACACACTTTACAATGAAGAA CGAAGGTTGGGGGATCAAAGAGGATCAGATACCCTCGTCGTCCCATTTTATACATCAAACGATGGGTTCT CACTCGACCAAAAACAAAAATATATTCGTATATTCGTATACGTTATATATCATATTCGATTTTCTAAGCT TTGCGCTCAATTTATATACGGTGAGATGTAAGTAATGGCTGCGTATTTATTACGTATGCTATATTATGAT GCACGCATTAGGCATGCGCTTACTGCAGAAATGTCTGAGATATTCCCCTCCGGGGGTAGTATGCACGCAA GTGTGAAACTTGAAGGAATTGACGGAAGGGCACCACAAGAACGCGTGGAGCATGTGGCTTAATTTGACTC AACGCGGGAAATCTTACCGGGTCCGGACACACTGAGGATTGACAGACAAACAACTATTAGTTGTGTACAA AGATGCTAGTTCTTTCATGATTATGTGATAGGTGGTGCATGGCCGTTCTTAGTTCGTGGAGTGATCTGTC TGCTTAATTGCGTTTCAATATTTAAATACGTATACGTATGACCCAATACATTGTATTGTGTGTCTAGTAT GCTATCATTTGAAAGCAACGAACGTGACCGTATCCTTTTATTAATATACTACACACGTATATATTCATTA TTAAAGGGACCGCTGTTTCTTTCTTTTAAACCAGAGGAAGGTTACGGCAATAACAGGTCTGTGATGCCCT TCGATGTTCCGGGCTGCACACGTGCTACAATGATTATTTCATTAAGTATCTCGTATATATGTGCATATAG CTCTTATATAAATCCTACCCTGAGAATGGGCGGGTAATCAATTAGAAGTAATGATTTCCTTTTTTTTATA CGCAACCATGTACCGTTATATTACATCCCACACATATTATTTATGCGTGCGTGTGTGTTTTATAAATTGT GTACGCGTAACTGTTAATTCATGGTGGGGATAGACCATTGTTAATTGTTGGTCTCGGTCTTAACTAGGAA TGCCTTGTACGGGTCATTGGTTCAACAAACCACCCGGAATACGTCCCTGCCCTTTGTACACACCGCCCGT CGCTCTTACCGATGAACTTCGCTATGAATCTATTGGACTGTACGCATGTACGGAAAGATATATGAATAGT GTGGTTTAAAGGAAAGAGAAGTCGTAACAAGGCATCAGTAGGTGAACCTGCTGATGGAT
Importance of organism and transcriptomes	Foraminifera are among the most abundant and diverse marine protists, characterized by granuloreticulopodia. Their tests or shells are an important component of marine sediments and widely used as biostratigraphic markers. Elphidium margaritaceum is classified within the order Rotaliida. The genus is one of the most common in the coastal waters and our strain was collected from the English Channel. To date, the molecular data on this organism, and foraminifers in general, is poorly represented in the available databases. Our knowledge on the gene expression repertoire and metabolic pathways used by foraminifers is still very limited. Additionally, transcriptomic data has shown to be a powerful tool for finding orthologous genes for further phylogenetic analysis, in an aim to resolve the phylogenetic relationships among the group and within the Eukaryotes.
Additional citations and references	i. Pillet L, De Vargas C, Pawlowski J. Molecular identification of sequestered diatom chloroplasts and kleptoplastidy in Foraminifera. Protist 2011;162:394-404.
Primary citation for organism's characterization, if available	Elphidium advenum (Cushman) var. margaritaceum Cushman, 1930, The foraminifera of the Atlantic Ocean; Part 7 - Nonionidae, Comerinida, Peneroplidae, Alveolinellidae. U.S. Nat. Mus., Bull., Washington, D.C., USA. No. 104, p.25
Sample collection site	English Channel
ENVO term for habitat - primary term	Acquatic: marine
ENVO term for habitat - secondary term	Sediment
Habitat	marine habitat
Country	FRANCE
Experimental Data
Date of experiment	06-DEC-12
Growth medium	non cultivable
Temperature (ºC)	18
Salinty (psu)	40
pH	8
Day portion of day:night cycle in hours	12
Night portion of day:night cycle in hours	12
Nitrate (μmol/L)	12
Ammonium (μmol/L)	4
Phosphate (μmol/L)	2
Silicate (μmol/L)	1
Trace elements (total) (nmol/L)	15
Prey organism, if applicable (genus and species)	Bacteria and diatoms
Investigation type	Eukaryotes