Chlamydomonas leiostraca SAG 11-49

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Environmental Data
Principle Investigator(s)	Alexandra Z. Worden
External sample ID	C_leios1149
NCGR Sample ID	MMETSP1391
Sample accession number	CAM_SMPL_003149
Assembly accession number	CAM_ASM_000867
Combined Assembly Name	N/A
Genus	Chlamydomonas
Species	leiostraca
Strain	SAG 11-49
Clonal	Unknown
Axenic	No
Prelim. NCBI Taxon ID	1034604
18S rRNA	TCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAACTGCTTATACTGTGAAACTGCGAATGGCTCAT TAAATCAGTTATAGTTTATTTGATGGTATACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCGTAAATCCCGACT TCTGGAAGGGACGTATTT-ATTAGATAAAAGGCCAGCCGGGCCGACCTTAGGCGAATCATGATAACTTCACGAATCGCAC GGCGCCGGCGATGTTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGAGGCCTACCATGGTGGTAACGGG TGACGGAGGATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAA ATTACCCAATCCCGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCGTCTGGTAATTGGAATGAGTACAATCTA AATCCCTTAACGAGTATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTA AGTTGTTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGTGGGTTCTAGCGGTCCGCCGTACTGCCCTTTCTGCCGGGGAC TGGGATTCATTTCTGGACTCGGAATCGGCGAGGTTACTTTGAGTAAATTAGTGTTCAAAGCAAGCCTACGCTCTGAATAC ATTAGCATGGAATAACACGATAGGACTCTGGCCTATCTTGTTGGTCTGTAGGACCGGAGTAATGATTAAGAGGGACAGTC GGGGGCATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACTTCTGCGAAAGCATTTGCCAAGG ATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATTAGATACCGTCGTAGTCTCAACCATAAACGATGCC GACTAGGGATTGGCAGGTGTTTCGTTGATGACCCTGCCAGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAG TATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGCGTGGAGCCTGGCTTAATTTGACTCAACA CGGGAAACTTACCAGGTCCAGACACGGGGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTGTGGGTGGTGGTGCATG GCCGTTCTTAGTTGGTGGGTTGCCTTGTCAGGTTGATTCCGGTAACGAACGAGACCTCAGCCTGCTAAATAGTCACGACT TCTTAGAGGGACTATTGACGTAGTCAATGGAAGTGTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGC ACGCGCGCTACACTGACGCATTCAACGAGCCTATCCTTGGCCGAGAGGTCCGGGTAATCTTTGAAACTGCGTCGTGATGG GGATAGATTATTGCAATTATTAGTCTTCAACGAGGAATGCCTAGTAAGCGAGTCATCAGCTCGCGTTGATTACGTCCCTG CCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGGGTGTGCTGGTGAAGTGTTCGGATTTGAGAAGATCATTAAACCC TCCCACCTAGAGGAAGGAGAAGTCGTAACAAGGTTTCC
Importance of organism and transcriptomes	Chlamydomonas leiostraca is a green alga closely related to the colorless chlorphyte Polytoma uvella SAG 62-2c. Comparative studies of these algal strains will provide relevant information to understand processes of genomic evolution associated to the loss of photosynthetic capabilities
Additional citations and references	Lang, I. et al. (2011) Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection. BMC Plant Biol 11:124
Primary citation for organism's characterization, if available	Ettl, H. (1958). Zur Kenntnis der Klasse Volvophyceae. In: Algologische Studien. (Komarek, J. & Ettl, H. Eds), pp. 207-289. Prague: Ceskoslov. Akad. Ved.
Collection date	01-JAN-51
Sample collection site	OTHER
ENVO term for habitat - primary term	Aquatic: freshwater
Habitat	freshwater habitat
Country	UNITED KINGDOM
Experimental Data
Date of experiment	27-OCT-11
Growth medium	SVM (Standard Volvox Media). Based on Kirk and Kirk (1983) Dev. Biol. 96:493?506
Modifications to growth medium	none
Temperature (ºC)	18
pH	8
Light (µmol photons / m2 / sec)	200
Day portion of day:night cycle in hours	14
Night portion of day:night cycle in hours	10
Nitrate (μmol/L)	500
Phosphate (μmol/L)	230
Total Fe (nmol/L)	2150
Trace elements (total) (nmol/L)	20
Urea (μmol/L)	500
Investigation type	Eukaryotes