Phaeocystis antarctica CCMP1374

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Environmental Data
Principle Investigator(s)	Mike Preston
External sample ID
NCGR Sample ID	MMETSP1444
Sample accession number	CAM_SMPL_003292
Assembly accession number	CAM_ASM_001103
Combined Assembly Name	N/A
Genus	Phaeocystis
Species	antarctica
Strain	CCMP1374
Clonal	Yes
Axenic	No
Prelim. NCBI Taxon ID	33657
18S rRNA	TTGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTTCTTTGATGGTACCTTGCTACTTGGATACCCGTAGTAATTCTAGAGCTAATACATGCAGGACTGCCCGACTTCGGAAGGGCTGTATTTATTAGATAAGAAACCATCTCGGGTCCGCCCGGTTGTGTGCTGAGTCATAATAACTGCTCGAATCGCACGGCTCTACGCCGGCGATGGTTCATTCAAATTTCTGCCCTATCAGCTTTCGATGGTAGGATAGAGGCCTACCATGGCGTTAACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAATGGCTACCACATCCAAGGAAGGCAGCAGGCGCGTAAATTGCCCGAATCCTGACACAGGGAGGTAGTGACAAGAAATAACAATACAGGGCTACTTCTAGTCTTGTAATTGGAATGAGTACAACTTACATCTCTTCACGAGGATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCGGATTTCGGGTCGGGCCGAGCGGTCTGCCGATGGGTATGCACTGTTTGGCGCGGCCTTCTTTCCGGAGACCGCGGCTACTCTTAACTGAGCGGGCGTGGGAGACGGATCGTTTACTTTGAAAAAATCAGAGTGTTTCAAGCAGGCAGCTCGCTCTTGCATGGATTAGCATGGGATAATTGAATAGGACTCTGGTGCTATTTTGTTGGTTTCGAACACCGGAGTAATGATTAACAGGGACAGTCAGGGGCACTCGTATTCCGTCGAGAGAGGTGAAATTCTCAGACCAATGGAAGACGAACCACTGCGAAAGCATTTGCCAGGGATGTTTTCACTGATCAAGAACGAAAGTTAGGGGATCGAAGACGATCAGATACCGTCGTAGTCTTAACCATAAACCATGCCGACTAGGGATTGGAGGGTGTTCGTTTCGACCTCTTCAGCACCTTACGGGAAACTAAAGTCTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCAACACGGGGAAACTTACCAGGTCCAGACATTGTTAGGATTGACAGATTGAGAGCTCTTTCTTGATTCGATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCGTAGCCTGCTAAATAGTTCCGCGAACTTCTTGTTGGCGGTCGACTTCTTAGAGGGACAACTTGTGACCAACAAGTGGAAGTTTGCGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGAAGCACTCAACGAGTCCACCTCGACCGACAGGTCTGGGAAACCTTTTGAAATTGCTTCGTGATGGGGATAGATTATTGCAACTATTAATCTTCAACGAGGAATTCCTAGTAAGCGCATGTCATCAGCGTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGCTCCTACCGATTGAATGATCCGGTGAGGCCCCCGGACTGGGGCGACGCATTCGGTTCGCCGGACGCGACGCTCCGGGAA
Importance of organism and transcriptomes	Important toxic antarctic species
Additional citations and references	Edvardsen, B., Eikrem, W., Green, J.C., Andersen, R.A., van der Stayy, S.Y.M. & Medlin, L. (2000). Phylogenetic reconstructions of the Haptophyta inferred from 18S ribosomal DNA sequences and available morphological data. Phycologia 39(1): 19-35.
Primary citation for organism's characterization, if available	Karsten, G. (1905). Das Phytoplankton des Antarktischen Meeres nach dem Material der deutschen Tiefsee-Expedition 1898-1899. Wissenschaftliche Ergebnisse der Deutschen Tiefsee-Expedition auf dem Dampfer 'Valdivia' 1898-1899 II 2: 1-136, pls 1-19.
Latitude	-77.8333
Longitude	-163
Depth (m)	10
Salinity (psu)	30
Temperature (ºC)	20
pH	7.9
Collection date	01-JAN-91
Sample collection site	Ross Sea
Sample material (e.g. "seawater," "sediment," etc.)	Seawater
ENVO term for habitat - primary term	Acquatic: marine
Other environmental metadata available	near sea ice
Other environmental metadata available	near sea ice
Habitat	marine habitat
Country	ANTARCTICA
Experimental Data
Date of experiment	25-AUG-13
Growth medium	L1 amended seawater
Temperature (ºC)	2
Salinty (psu)	33
pH	8.2
Light (µmol photons / m2 / sec)	100
Day portion of day:night cycle in hours	13
Night portion of day:night cycle in hours	11
Nitrate (μmol/L)	882
Phosphate (μmol/L)	36.2
Silicate (μmol/L)	106
Total Fe (nmol/L)	11.7
Trace elements (total) (nmol/L)	12871.2
CO2 (ppm)	390
Pressure (atm)	1
Investigation type	Eukaryotes